[1] Alvisi, G., Jans, D., Guo, J., Pinna, L., and Ripalti, A. (2005) A protein kinase CK2 site flanking the nuclear targeting signal enhances nuclear transport of human cytomegalovirus ppUL44, Traffic 6, 1002-1013.

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[2] Wagstaff, K. M., Dias, M. M., Alvisi, G., and Jans, D. A. (2005) Quantitative analysis of protein-protein interactions by native page/fluorimaging, J Fluoresc 15, 469-473.

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[3] Alvisi, G.*, Jans, D., and Ripalti, A. (2006) Human cytomegalovirus (HCMV) DNA polymerase processivity factor ppUL44 dimerizes in the cytosol before translocation to the nucleus, Biochemistry 45, 6866-6872.

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[4] Alvisi, G., Poon, I. K., and Jans, D. A. (2006) Tumor-specific nuclear targeting: promises for anti-cancer therapy?, Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 9, 40-50.

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[5] Alvisi, G.*, Ripalti, A., Ngankeu, A., Giannandrea, M., Caraffi, S. G., Dias, M. M., and Jans, D. A. (2006) Human cytomegalovirus DNA polymerase catalytic subunit pUL54 possesses independently acting nuclear localization and ppUL44 binding motifs, Traffic 7, 1322-1332.

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[6] Alvisi, G.*, Musiani, D., Jans, D. A., and Ripalti, A. (2007) An importin alpha/beta-recognized bipartite nuclear localization signal mediates targeting of the human herpes simplex virus type 1 DNA polymerase catalytic subunit pUL30 to the nucleus, Biochemistry 46, 9155-9163.

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[7] Alvisi, G.*, Avanzi, S., Musiani, D., Camozzi, D., Leoni, V., Ly-Huynh, J. D., and Ripalti, A. (2008) Nuclear import of HSV-1 DNA polymerase processivity factor UL42 is mediated by a C-terminally located bipartite nuclear localization signal, Biochemistry 47, 13764-13777.

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[8] Alvisi, G., Rawlinson, S. M., Ghildyal, R., Ripalti, A., and Jans, D. A. (2008) Regulated nucleocytoplasmic trafficking of viral gene products: a therapeutic target?, Biochim Biophys Acta 1784, 213-227.

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[9] Kuusisto, H. V., Wagstaff, K. M., Alvisi, G.#, and Jans, D. A.# (2008) The C-terminus of apoptin represents a unique tumor cell-enhanced nuclear targeting module, International journal of cancer. Journal international of cancer 123, 2965-2969.

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[10] Sinigalia, E.#,Alvisi, G.#, Mercorelli, B., Coen, D. M., Pari, G. S., Jans, D. A., Ripalti, A., Palu, G., and Loregian, A. (2008) Role of homodimerization of human cytomegalovirus DNA polymerase accessory protein UL44 in origin-dependent DNA replication in cells, J Virol 82, 12574-12579.

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[11] Alvisi, G., Kuusisto, H. V., Jans, D. A., and Wagstaff, K. M. (2009) The tumor cell specific nuclear targeting signal of Apoptin, In Proteins Killing Tumour Cells(Backendorf, C., Noteborn , M. H. M., and Tavassoli, M., Eds.), p 11, Research-Signpost, Trivandrum.

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[12] Alvisi, G.*, Roth, D. M., Camozzi, D., Pari, G. S., Loregian, A., Ripalti, A., and Jans, D. A. (2009) The flexible loop of the human cytomegalovirus DNA polymerase processivity factor ppUL44 is required for efficient DNA binding and replication in cells, J Virol 83, 9567-9576.

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[13] Fulcher, A. J., Roth, D. M., Fatima, S., Alvisi, G., and Jans, D. A. (2010) The BRCA-1 binding protein BRAP2 is a novel, negative regulator of nuclear import of viral proteins, dependent on phosphorylation flanking the nuclear localization signal, FASEB J 24, 1454-1466.

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[14] Gaibani, P., Pellegrino, M. T., Rossini, G., Alvisi, G., Miragliotta, L., Prati, C., and Sambri, V. (2010) The central region of the msp gene of Treponema denticola has sequence heterogeneity among clinical samples, obtained from patients with periodontitis, BMC Infect Dis 10, 345.

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[15] Alvisi, G.#, Madan, V.#, and Bartenschlager, R. (2011) Hepatitis c virus and host cell lipids An intimate connection, Rna Biology 8, 258-269.

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[16] Alvisi, G.*, Marin, O., Pari, G., Mancini, M., Avanzi, S., Loregian, A., Jans, D. A., and Ripalti, A. (2011) Multiple phosphorylation sites at the C-terminus regulate nuclear import of HCMV DNA polymerase processivity factor ppUL44, Virology 417, 259-267.

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[17] Kuusisto, H. V., Wagstaff, K. M., Alvisi, G., Roth, D. M., and Jans, D. A. (2012) Global enhancement of nuclear localization-dependent nuclear transport in transformed cells, FASEB J 26, 1181-1193.

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[18] Pichlmair, A., Kandasamy, K., Alvisi, G., Mulhern, O., Sacco, R., Habjan, M., Binder, M., Stefanovic, A., Eberle, C. A., Goncalves, A., Burckstummer, T., Muller, A. C., Fauster, A., Holze, C., Lindsten, K., Goodbourn, S., Kochs, G., Weber, F., Bartenschlager, R., Bowie, A. G., Bennett, K. L., Colinge, J., and Superti-Furga, G. (2012) Viral immune modulators perturb the human molecular network by common and unique strategies, Nature 487, 486-490.

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[19] Ruggieri, A., Dazert, E., Metz, P., Hofmann, S., Bergeest, J. P., Mazur, J., Bankhead, P., Hiet, M. S., Kallis, S., Alvisi, G., Samuel, C. E., Lohmann, V., Kaderali, L., Rohr, K., Frese, M., Stoecklin, G., and Bartenschlager, R. (2012) Dynamic oscillation of translation and stress granule formation mark the cellular response to virus infection, Cell Host Microbe 12, 71-85.

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[20] Sinigalia, E.#, Alvisi, G.#, Segre, C. V.#, Mercorelli, B.#, Muratore, G., Winkler, M., Hsiao, H. H., Urlaub, H., Ripalti, A., Chiocca, S., Palu, G., and Loregian, A. (2012) The human cytomegalovirus DNA polymerase processivity factor UL44 is modified by SUMO in a DNA-dependent manner, PLoS One7, e49630.

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[21] Alvisi, G.*, and Palu, G.* (2013) Reprogramming the host: Modification of cell functions upon viral infection, World J Virol 2, 16-17.

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[22] Amberkar, S., Kiani, N. A., Bartenschlager, R., Alvisi, G.*, and Kaderali, L. (2013) High-throughput RNA interference screens integrative analysis: Towards a comprehensive understanding of the virus-host interplay, World J Virol 2, 18-31.

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[23] Avanzi, S., Alvisi, G., and Ripalti, A. (2013) How virus persistence can initiate the tumorigenesis process, World J Virol 2, 102-109.

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[24] Avanzi, S., Leoni, V., Rotola, A., Alviano, F., Solimando, L., Lanzoni, G., Bonsi, L., Di Luca, D., Marchionni, C., Alvisi, G., and Ripalti, A. (2013) Susceptibility of human placenta derived mesenchymal stromal/stem cells to human herpesviruses infection, PLoS One 8, e71412.

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[25] Di Iorio, E., Barbaro, V., Del Vecchio, C., Alvisi, G., Poletti, V., Parekh, M., Palù, G., and Parolin, C. (2013) Gene therapy approaches for corneal diseases, In New Insight on Biobanks (Caenazzo, L., Ed.).

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[26] Alvisi, G.,*Jans, D. A., Camozzi, D., Avanzi, S., Loregian, A., Ripalti, A., and Palu, G.* (2013) Regulated transport into the nucleus of herpesviridae DNA replication core proteins, Viruses 5, 2210-2234

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[27] Alvisi, G.*, and Jans, D. A.* (2014) Comment on Phosphorylation adjacent to the nuclear localization signal of human dUTPase abolishes nuclear import: structural and mechanistic insights by Rona et al. (2013), Acta crystallographica. Section D, Biological crystallography 70, 2775-2776.

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[28] Eberle, C. A., Zayas, M., Stukalov, A., Pichlmair, A., Alvisi, G., Muller, A. C., Bennett, K. L., Bartenschlager, R., and Superti-Furga, G. (2014) The lysine methyltransferase SMYD3 interacts with hepatitis C virus NS5A and is a negative regulator of viral particle production, Virology 462-463, 34-41.

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[29] Alvisi, G., and Jans, D. A. (2015) Basis of cargo recognition by importin alphas: the power of structure, Structure 23, 251-252.

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[30] Alvisi, G., and Jans, D. A. (2015) Regulating post-mitotic nuclear access: Cdk1-phosphorylation of NLSs, Cell cycle 14, 695-696.

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[31] Andrisani, A., Dona, G., Tibaldi, E., Brunati, A. M., Sabbadin, C., Armanini, D., Alvisi, G., Gizzo, S., Ambrosini, G., Ragazzi, E., and Bordin, L. (2015) Astaxanthin Improves Human Sperm Capacitation by Inducing Lyn Displacement and Activation, Mar Drugs 13, 5533-5551.

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[32] Bonamassa, B., Ciccarese, F., Antonio, V. D., Contarini, A., Palu, G., and Alvisi, G.*(2015) Hepatitis C virus and host cell nuclear transport machinery: a clandestine affair, Front Microbiol 6, 619.

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[33] Metz, P., Chiramel, A., Chatel-Chaix, L., Alvisi, G., Bankhead, P., Mora-Rodriguez, R., Long, G., Hamacher-Brady, A., Brady, N. R., and Bartenschlager, R. (2015) Dengue Virus Inhibition of Autophagic Flux and Dependency of Viral Replication on Proteasomal Degradation of the Autophagy Receptor p62, J Virol89, 8026-8041.

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[34] Alvisi, G.*, and Jans, D. A.* (2016) Secret life of importin-beta; solenoid flexibility as the key to transport through the nuclear pore, Acta Crystallogr D Struct Biol72, 703-704.

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[35] Alvisi, G.*, Paolini, L., Contarini, A., Zambarda, C., Di Antonio, V., Colosini, A., Mercandelli, N., Timmoneri, M., Palu, G., Caimi, L., Ricotta, D., and Radeghieri, A.* (2018) Intersectin goes nuclear: secret life of an endocytic protein, The Biochemical journal 475(8):1455-72

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[36] Alvisi, G., Trevisan, M., Masi, G., Canel, V., Caenazzo, L., Nespeca, P., Barzon, L., Di Iorio, E., Barbaro, V., and Palu, G. (2018) Generation of a transgene-free human induced pluripotent stem cell line (UNIPDi001-A) from oral mucosa epithelial stem cells, Stem Cell Res 28, 177-180.

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[37] Raza S, Alvisi G, Shahin F, Husain U, Rabbani M, Yaqub T, Anjum AA, Sheikh AA, Nawaz M, Ali MA. (2018) Role of Rab GTPases in HSV-1 infection: Molecular understanding of viral maturation and egress. Microb Pathog. 118:146-53.

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[38] Reale, A., Trevisan, M., Alvisi, G., and Palu, G. (2018) The silent enemy: Celiac disease goes viral, Journal of cellular physiology 233, 2693-2694.

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[39] Trevisan, M., Barbaro, V., Riccetti, S., Masi, G., Barzon, L., Nespeca, P., Alvisi, G., Di Iorio, E., and Palu, G. (2018) Generation of a transgene-free induced pluripotent stem cells line (UNIPDi002-A) from oral mucosa epithelial stem cells carrying the R304Q mutation in TP63 gene, Stem Cell Res 28, 149-152.

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[40] Trevisan, M., Di Antonio, V., Radeghieri, A., Palu, G., Ghildyal, R., and Alvisi, G.*(2018) Molecular Requirements for Self-Interaction of the Respiratory Syncytial Virus Matrix Protein in Living Mammalian Cells, Viruses 10(3).

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[41] Trevisan, M., Di Iorio, E., Masi, G., Riccetti, S., Barzon, L., Alvisi, G., Caenazzo, L., Barbaro, V., and Palu, G. (2018) Induced pluripotent stem cells line (UNIPDi003-A) from a patient affected by EEC syndrome carrying the R279H mutation in TP63 gene, Stem Cell Res 28, 141-144.

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[42] Smith KM, Di Antonio V, Bellucci L, Thomas DR, Caporuscio F, Ciccarese F, Ghassabian H, Wagstaff KM, Forwood JK, Jans DA, Palu G, Alvisi G.* (2018) Contribution of the residue at position 4 within classical nuclear localization signals to modulating interaction with importins and nuclear targeting. Biochim Biophys Acta 1865(8):1114-29.

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[43] Trevisan M#, Alvisi G#, Barbaro V, Barzon L, Raffa P, Migliorati A, Desole G, Ruzittu S, Masi G, Di Iorio E, Palu G. (2018) Oral Mucosa-Derived Induced Pluripotent Stem Cells from Patients with Ectrodactyly-Ectodermal Dysplasia-Clefting Syndrome. Cell Reprogram. 

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[44] Salata C, Calistri A, Alvisi G, Celestino M, Parolin C, Palu G. (2019) Ebola Virus Entry: From Molecular Characterization to Drug Discovery. Viruses. 11(3).

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[45] Di Iorio E, Barbaro V, Alvisi G, Trevisan M, Ferrari S, Masi G, Nespeca P, Ghassabian H, Ponzin D, Palu G. (2019). New Frontiers of Corneal Gene Therapy. Hum Gene Ther doi:10.1089/hum.2019.026.

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[46] Salvi A, Vezzoli M, Busatto S, Paolini L, Faranda T, Abeni E, Caracausi M, Antonaros F, Piovesan A, Locatelli C, Cocchi G, Alvisi G, De Petro G, Ricotta D, Bergese P, Radeghieri A. (2019). Analysis of a nanoparticle enriched fraction of plasma reveals miRNA candidates for Down syndrome pathogenesis. Int J Mol Med 43:2303-2318.

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[47] Elbadawy HM, Abdul MIM, Aljuhani N, Vitiello A, Ciccarese F, Shaker MA, Eltahir HM, Pala G, Di Antonio V, Ghassabian H, Del Vecchio C, Salata C, Franchin E, Ponterio E, Bahashwan S, Thabet K, Abouzied MM, Shehata AM, Parolin C, Calistri A, Alvisi, G*. (2020). Generation of Combinatorial Lentiviral Vectors Expressing Multiple Anti-Hepatitis C Virus shRNAs and Their Validation on a Novel HCV Replicon Double Reporter Cell Line. Viruses-Basel 12.

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[48] Raza S, Shahin F, Zhai W, Li H, Alvisi G, Yang K, Chen X, Chen Y, Chen J, Hu C, Chen H, Guo A. (2020). Ivermectin Inhibits Bovine Herpesvirus 1 DNA Polymerase Nuclear Import and Interferes with Viral Replication. Microorganisms 8.

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[49] Di Antonio V, Palu G, Alvisi G.* (2021). Live-Cell Analysis of Human Cytomegalovirus DNA Polymerase Holoenzyme Assembly by Resonance Energy Transfer Methods. Microorganisms 9.

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[50] Ghassabian H, Falch F, Timmoner M, Mercorelli B, Loregian A, Palù G, Alvisi G.* (2021). Divide et impera: An In Silico Screening Targeting HCMV ppUL44 Processivity Factor Homodimerization Identifies Small Molecules Inhibiting Viral Replication. Viruses 13.

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[51] Dorigatti I, Lavezzo E, Manuto L, Ciavarella C, Pacenti M, Boldrin C, Cattai M, Saluzzo F, Franchin E, Del Vecchio C, Caldart F, Castelli G, Nicoletti M, Nieddu E, Salvadoretti E, Labella B, Fava L, Guglielmo S, Fascina M, Grazioli M, Alvisi G, Vanuzzo MC, Zupo T, Calandrin R, Lisi V, Rossi L, Castagliuolo I, Merigliano S, Unwin HJT, Plebani M, Padoan A, Brazzale AR, Toppo S, Ferguson NM, Donnelly CA, Crisanti A.  (2021) SARS-CoV-2 antibody dynamics and transmission from community-wide serological testing in the Italian municipality of Vo'. Nat Commun. Jul 19;12(1):4383. 

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[52] Messa L, Celegato M, Bertagnin C, Mercorelli B, Alvisi G, Banks L, Palù G, Loregian A. (2021) The Dimeric Form of HPV16 E6 Is Crucial to Drive YAP/TAZ Upregulation through the Targeting of hScrib. Cancers (Basel). Aug 13;13(16)

 

[53]  Barbaro V, Orvieto A, Alvisi G, Bertolin M, Bonelli F, Liehr T, Harutyunyan T, Kankel S, Joksic G, Ferrari S, Daniele E, Ponzin D, Bettio D, Salviati L, Di Iorio E. Analysis and pharmacological modulation of senescence in human epithelial stem cells. J Cell Mol Med 2022, 26, (14), 3977-3994.

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[54] Centazzo M, Manganaro L, Alvisi G (*). Cellular Targets of HIV-1 Protease: Just the Tip of the Iceberg? Viruses. 2023;15(3).

 

[55] Alvisi G (*), Manaresi E, Cross EM, Hoad M, Akbari N, Pavan S, et al. Importin alpha/beta-dependent nuclear transport of human parvovirus B19 nonstructural protein 1 is essential for viral replication. Antiviral Res. 2023;213:105588. 

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[56] Cross, E.M., Akbari, N., Ghassabian, H., Hoad, M., Pavan, S., Ariawan, D., Donnelly, C.M., Lavezzo, E., Petersen, G.F., Forwood, J.K. & Alvisi, G. (*), 2023. A functional and structural comparative analysis of Large Tumor Antigens reveals evolution of different importin alpha-dependent nuclear localization signals. Protein Sci. https://doi.org/10.1002/pro.4876.

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[57] Cross, E.M., Marin, O., Ariawan, D., Aragao, D., Cozza, G., Di Iorio, E., Forwood, J.K. & Alvisi, G. (*), 2023. Structural determinants of phosphorylation-dependent nuclear transport of HCMV DNA polymerase processivity factor UL44. FEBS Lett. https://doi.org/10.1002/1873-3468.14797.

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* = corresponding author; # = shared authorship